Chrysin-Mediated Promotion of Odontoblastic Differentia- tion: A Pathway Towards Dental Pulp Regeneration

Abstract

Dental pulp, a critical component in tooth development and maintenance, plays a vital role in dentin formation, nutrient supply, and signal transmission. When damaged, pulp can become infected and necrotic, leading to pain and a decline in the quality of life. Current clinical treatments, such as root canal therapy, though effective, result in the loss of pulp vitality and associated sensory and defensive functions. Recognizing the potential of regenerative approaches, our study explored the odontogenic differentiation effects of Chrysin, a natural flavonoid, on human dental pulp cells (hDPCs). Our investigations revealed that Chrysin, particularly at a concentration of 10 μM, exhibited no cytotoxic effects on hDPCs over a 5-day period. Notably, this concentration demonstrated optimal effects on alkaline phosphatase (ALP) activity and mineralization capacity, indicating its potential as an agent for odontogenic differentiation. Further analyses at the molecular level confirmed increased expression of odontogenic markers, including dentin matrix protein 1 (DMP-1), dentin sialophosphoprotein (DSPP), and ALP, supporting the pro-odontogenic effects of chrysin. In conclusion, our study underscores the positive effects of Chrysin on odontogenic differentiation in hDPCs, offering a promising avenue for further exploration in dental regenerative medicine. Future research should delve into in vivo applications and clinical assessments to validate the translational impact of Chrysin in preserving the vital pulp and advancing dental tissue regeneration.